Sue Lunte Research Group

Facilities

MRB photo

The Multidisciplinary Research Buildings is a state-of-the-art, three-story research facility completed and occupied in January 2006. Laboratory spaces on the outside of the building wrap around a shared core of research equipment and facilities, including bio-safety level 3 labs, a mass spectrometry lab and "clean" rooms.

Microfabrication Facilities, located on the first floor of the MRB, contains 2,400 ft2 of cleanroom space, 900 ft2 for a machining room, 400 ft2 of wet lab space and offices. This facility is under the direction of Drs. Susan Lunte and Karen Nordheden. The facility is available to KU researchers in all departments and provides training to new investigators and graduate students in the use of microfabrication procedures and equipment. In the long-term, the center will evolve into a self-supporting facility of service to the wider University of Kansas community.

The Microfabrication Facility cleanrooms consist of a gowning vestibul class="spaced"e (200 ft2); a class 100 cleanroom for imaging (190 ft2); a class 100 area for photolithography (200 ft2); a class 1000 space for metal deposition (280 ft2); a class 1000 space for plasma etching (280 ft2); and a class 1000 wet lab for substrate etching, bonding, and device inspection (575 ft2). The facility has access corridors for gas tanks, house nitrogen, specialized hoods and lighting, and built in desiccators. The facility provides capabilities for photolithography, plasma etching of high surface ratio elements, glass etching, laser ablation, metal deposition, dielectric material deposition and microchip characterization. The major equipment in this facility is listed in the equipment section below.

General Laboratory equipment

microchip CE system

Ted running microchip CE system.

Capillary electrophoresis systems

  • Hewlett-Packard CE system capable of interfacing with an EC detector
  • ABI CE systems
  • ISCO capillary electrophoresis systems

Liquid Chromatography

  • Spectra Physics SP8800
  • BAS PM-80 with gradient Microbore capability
  • Shimadzu LC-6A

U. V. detectors

  • Perkin-Elmer LC90UV
  • Waters 484
  • Applied Biosystems detectors
the LC-MS

Josh and Arvind using LC-MS.

Laser systems

  • Kimmon 1K He-Cd lasers (325, 442nm)
  • Liconix He-CD laser (442nm)
  • Ar-ion laser (488 nm)

Electrochemistry

  • BAS LC-4B and 3 BAS LC-4C potentiostats
  • Bioanalytical electrochemical analyzer
  • Dionex ED 40 EC analyzer
  • BAS CV-50W, C3 - for cyclic voltametry

A Hewlett-Packard G2025A MALDI-TOF Mass Spectrometer is also available, plus access to the departmental Waters 2690 LC system coupled to a Micromass Quattro micro tandem mass spectrometer.

Microfabrication facility

etching station

Van prepares glass chip using HF etching station.

The microfabrication facility is located in 670 sq. ft. of laboratory space on the first floor of the MRB. The facility includes a 120 sq. ft. class-1000 clean room that houses the basic photolithography equipment needed for production of the microchip analytical systems including:


  • Brewer Science spin coater
  • Terra Universal hood
  • OAI Autoflood UV source
  • Digitally controlled hot plate
  • Sterotaxic light microscope
  • CVC thermal evaporator equipped with a CTI cryogenic pump and Inficon deposition monitor
  • Universal CO2 laser system for ablating plastics
  • Fisher Scientific programmable furnace
  • Thelco/GCA precision oven
  • Lesker thin film deposition system
  • Denton Vacuum carbon sputterer
  • Herrick plasma cleaner
  • Tencor Alpha Step 100 profilometer
  • Lindberg blue tube furnace
  • HF etching station
thin film deposition system

Nate fabricates metal electrodes using Lesker thin film deposition system.

Evaluation of fabricated devices is performed with some of the following equipment:

  • BAS potentiostats
  • 0-30-kV power supplies
  • 2-kV miniature power supplies (KU IDL)
  • Dell computers, with epsilon EC detectors
  • Jenway multi-output high voltage power supplies for microchips
  • Gateway computer systems with BAS data acquisition interfaces
  • Carl Zeiss Axiolab microscope equipped with fluorescence accessories, CCD camera and video monitor, and Dell computer with video capture card

Instrument Design Lab

The University of Kansas has an instrument design laboratory that can assist with the development of specialized potentiostats and data acquisition devices. The IDL designs and fabricates electronic and computer-based instrumentation as well as programmable mechanical devices that interact with the computer. It is fully equipped with test equipment and fabrication equipment, as well as significant stocks of integrated circuits, passive components, connectors, etc.

Tissue Culture Laboratory

LIF detection

Bryan inspects microchip system with LIF detection.

This facility occupies ~2,000 sq. ft. of space in Malott Hall. The Tissue Culture component is a ~1,000 sq. ft. positive pressure lab totally designed for preparation and maintenance of cell cultures. The ante-room is designed for media preparation and is equipped with a large autoclave, water purification system, and several refrigerators and freezers, including a -80 degree C freezer. University lab staff members have access to all of the instrumentation, based on a sign-up system to reserve items for use as needed. The Facility is under the direction of an experienced tissue culture expert, who handles maintenance of the equipment and efficient utilization of the facility.

The Tissue Culture/Hybridoma Facility is equipped with 4 NuAire and Baker tissue culture hoods, 8 Forma and NuAire CO2 incubators, 1 double-chamber trigas incubator, a coulter counter, bioreactors, liquid N2 tanks for cell preservation, centrifuges, several microscopes, balances, and other small equipment items required for cell culture. Other components of the facility include Packard and Biotek UV/VIS, fluorescence, and chemiluminescence plate readers, a Becton Dickinson FACScan flow cytometer with two workstations, and a Nikon TE200 fluorescence microscope with video camera, imaging system, and computer.


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